Targets of the Entamoeba histolytica Transcription Factor URE3-BP

The Entamoeba histolytica transcription factor Upstream Regulatory Element 3-Binding Protein (URE3-BP) is a calcium-responsive regulator of two E. histolytica virulence genes, hgl5 and fdx1. URE3-BP was previously identified by a yeast one-hybrid screen of E. histolytica proteins capable of binding to the sequence TATTCTATT (Upstream Regulatory Element 3 (URE3)) in the promoter regions of hgl5 and fdx1. In this work, precise definition of the consensus URE3 element was performed by electrophoretic mobility shift assays (EMSA) using base-substituted oligonucleotides, and the consensus motif validated using episomal reporter constructs. Transcriptome profiling of a strain induced to produce a dominant-positive URE3-BP was then used to identify additional genes regulated by URE3-BP. Fifty modulated transcripts were identified, and of these the EMSA defined motif T[atg]T[tc][cg]T[at][tgc][tg] was found in over half of the promoters (54% p<0.0001). Fifteen of the URE3-BP regulated genes were potential membrane proteins, suggesting that one function of URE3-BP is to remodel the surface of E. histolytica in response to a calcium signal. Induction of URE3-BP leads to an increase in tranwell migration, suggesting a possible role in the regulation of cellular motility

From Interactions to Institutions: Microprocesses of Framing and Mechanisms for the Structuring of Institutional Fields

Despite the centrality of meaning to institutionalization, little attention has been paid to how meanings evolve and amplify to become institutionalized cultural conventions. We develop an interactional framing perspective to explain the microprocesses and mechanisms by which this occurs. We identify three amplification processes and three ways frames stack up or laminate that become the building blocks for diffusion and institutionalization of meanings within organizations and fields. Although we focus on “bottom-up” dynamics, we argue that framing occurs in a politicized social context and is inherently bidirectional, in line with structuration, because microlevel interactions instantiate macrostructures. We consider how our approach complements other theories of meaning making, its utility for informing related theoretical streams, and its implications for organizing at the meso and macro levels

Weapons, Strategies, and Tactics of the Europeans and the Indians in Sixteenth- and Seventeenth-Century Florida

The Indians of Florida were hostile to white explorers, adventurers, colonists, and missionaries from the time of Ponce de León’s encounter in 1513 until 1710 when it was reported, “there remains not now so much as one Village with ten Houses in it, in all Florida, that is subject to the Spaniards.“ These were the Apalachee and Timucua Indians of northwest and central Florida, but it is known that the Tocobago Indians had been destroyed by 1709. The Ais, never many in number, seem to have disappeared sometime during the first half of the eighteenth century, and the Tekesta were finally exterminated by bands of raiding Creeks. Some historians believe the last remnants of these and the Calusa Indians went to Havana with the Spaniards in 1763, but there is evidence that a few Calusa Indians remained near Charlotte Harbor and later supported the Seminoles

Enduring records The environmental and cultural heritage of wetlands

Includes bibliographical referencesAvailable from British Library Document Supply Centre- DSC:9261. 86732(15) / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo